Influence of Formaldehyde in the Fluorescence Formation Related to Fish Deterioration
نویسنده
چکیده
In previous studies fluorescence detection was accomplished at different excitation/emission maxima during common fish processing. A bathochromic shift towards higher wavelength maxima was observed and measured as the ratio between absorption at two of the maxima tested. This fluorescence ratio (δF) value correlated with increased fish damage. In the present work, the influence of formaldehyde (FA) in the formation of the same δF value was studied. For it, FA reacted in model systems at 30°C during 25 days with propylamine and fish muscle. It was observed that FA showed a lower ability in producing fluorescent compounds than the common fish oxidation products (propanal and hexanal) that were also tested. However, in the presence of both lipid oxidation aldehydes, the FA containing mixtures produced a higher δF value. Model systems consisting of FA and fatty fish (sardine) muscle produced a higher fluorescence development than in the case of FA and lean fish (cod), as a result of a greater presence in the first case of lipid oxidation compounds formed during the reaction conditions. From the muscle systems results, it is concluded that the presence of FA in a reacting medium enhances the fluorescence formation, so that the δF value could be an accurate tool in order to assess fish damage. This determination could be profitable in processes such as gadoid fish freezing where both the FA and lipid oxidation are produced. Running title: Formaldehyde and fluorescence formation.
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